yhxA [2019-05-09 17:43:33]

similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase

locus: BSU09260

pI: 5.64

mw: 49.71 kDa

protein length: 450 aa

gene length: 1350 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,000,364 → 1,001,716

The protein

Protein family

• class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|13DC4296015A28975BC7697F134FB6ECD6B11489|YodT]:
• [protein|68C86FED3575B864C576DFB73FE7B5BF83994D1F|BioA]:
• [protein|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|GabT]:

[SW|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|3N5M] (from ''Bacillus Anthracis'', 64% identity)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA]-[gene|38767691AE7E09F46B9E97A60BF5358C1876EDF8|glpP]
  description: [Pubmed|2127799]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21925382], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  additional information

  • half-life of the mRNA: 3.7 min [PubMed|21925382]
  • half-life of the mRNA: 3.7 min [PubMed|21925382]

Biological materials

Mutant

• MGNA-B475 (yhxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1474 NBRP B. subtilis, Japan]
• BKE09260 (Δ[gene|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTAGCGTTCCCTA, downstream forward: _UP4_TAATATAAAAGTGAATTATT
• BKK09260 (Δ[gene|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTAGCGTTCCCTA, downstream forward: _UP4_TAATATAAAAGTGAATTATT

References

• 17981983,2127799,21925382